P6887 | CAS:9001-75-6 | Pepsin A | Pepsin from porcine gastric mucosa | 胃蛋白酶

P6887  Pepsin from porcine gastric mucosa Sigma      lyophilized powder, 3,200-4,500 units/mg protein 窗体底端 产品 号 您的价格 RMB P6887-250MG 522.99 P6887-1G 1,387.62 P6887-5G 4,220.19 P6887-10G 7,276.23 P6887-250G 85,590.18 Synonym: Pepsin A, Pepsin from hog stomach CAS Number: 9001-75-6 Enzyme Commission (EC) Number: 3.4.23.1  ( BRENDA | IUBMB ) EC Number: 232-629-3 MDL number: MFCD00081840 Description Analysis Note E1%/280=14.7   Protein determined by E1%/280   Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5. Application Used to produce F(ab′)2 fragments of antibodies. Biochem/physiol Actions Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin Other Notes View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer. Unit Definition One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 ml. Light path = 1 cm.) 性质 form lyophilized powder mol wt mol wt 35 kDa total impurities salt, essentially free EQP level Premium storage temp. −20°C 安全性 Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves Hazard Codes Xn Risk Statements 36/37/38-42 Safety Statements 22-24-26-36/37 WGK Germany 1 RTECS SC6132000