L8754 | PHA-P | Phytohemagglutinin P | 植物血球凝集素 P

L8754   Lectin from Phaseolus vulgaris (red kidney bean) Sigma       Phytohemagglutinin PHA-P, lyophilized powder 货号 号 目录价 RMB L8754-5MG 237.51 L8754-25MG 675.09 L8754-50MG 1,157.13 Synonym: Phaseolus vulgarisagglutinin, PHA EC Number: 232-718-7 Description Analysis Note Agglutination activity is expressed in mg/ml and is determined from serial dilutions of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a suspension of 2% human erythrocytes in phosphate buffered saline, pH 6.8, after 1 hr incubation at 25 °C. Mitogenic activity is determined by BrdU incorporation in HeLa cell cultures. Biochem/physiol Actions PHA consists of two molecular species, an erythroagglutinin (PHA-E) which has low mitogenic activity and high erythroagglutinin activity, and leucoagglutinin (PHA-L) which has high mitogenic and leucoagglutinating activity, but very low erythroagglutinating activity.1,2 PHA-E is not blood group specific, but agglutination can be inhibited by certain oligosaccharides.3 PHA-P is the protein form of PHA prior to separation and purification of erythroagglutinin and leucoagglutinin.4 PHA-M is the mucoprotein form.4 Conjugates are prepared from the corresponding purified lectins. 性质 form lyophilized powder potency <62.5 μg per mL agglutination activity   <10 μg/mL mitogenic activity total impurities salt, free storage temp. 2-8°C 安全性 Safety Statements 22-24/25 RTECS TJ3470000