拟南芥的种植 Silwet l-77

拟南芥的种植 Silwet l-77 拌土：蛭石:黑土:珍珠岩=3:1:0.5用拟南芥PNS营养液浇，然后再将拟南芥种子点到土上。再用保鲜膜封住，再用报纸盖上数日后取走报纸，每天光照16小时。 拟南芥PNS营养液               1M 磷酸缓冲液（PH5.5）   2.5ml               1M KNO3                              5ml               1M MgSO4·7H2O                  2ml                1M Ca(NO3)2·4 H2O             2ml               20mM Fe·EDTA                  2.5ml               MS微量元素                           1ml               加水定容至1L   转化试剂Silwet L-77 拟喃芥、油菜、大肠杆菌等转化必须试剂之一，原产地为美国GE公司. Silwet L-77 is a chemical mixture of polyalkyleneoxide modified heptamethyltrisiloxane (84%) and allyloxypolyethyleneglycol methyl ether (16%). It is an essential infiltration media ingredient for all Agrobacterium-based transformations of Arabidopsis and other plants. 10 mL and 35 mL are sufficient quantity to prepare 33 L and 115 L of vacuum infiltration medium when used at the recommended rate of 0.03%, v/v, respectively. SILWET L-77 is a unique surfactant designed to improve the spreading and wetting properties of pesticide spray mixes. SILWET L-77 utilizes unique organosilicone chemistry which provides the most complete coverage possible. SILWET L-77 can improve cuticular penetration of plant surfaces by the spray mix. Optimum application and resultant effects can be influenced by many factors. Consequently, it is recommended that careful observation of the spray deposit be made and adjuvant rates adjusted accordingly. Applications should be made to ensure thorough coverage without excessive run-off. TYPICAL PROPERTIES Physical appearance Clear, light amber liquid Pounds per gallon 8.370 Specific gravity 1.0 - 1.01 pH (1.0%) 6.0 - 7.0 Freeze Point less than 35 degrees F Surface Tension (0.125% v/v) less than 25 dynes/cm Contact Angle (0.125% v/v) less than 5 degrees F Draves Wet Time (0.125% v/v) less than 15.0 seconds GENERAL PRODUCT INFORMATION (1) SILWET L-77 contains organosilicone surfactants designed to provide superior wetting and coverage. (2) SILWET L-77 can improve water penetration of hard to wet soils and provide more uniform distribution of applied moisture. (3) SILWET L-77 is generally compatible with pesticides and fertilizer solutions. (4) SILWET L-77 may cause excessive foam if not properly added to the spray tank. To minimize foam generation, add SILWET L-77 to the tank after all other ingredients are added. If foaming is still a concern, add an antifoaming agent, such as FOAMBUSTER or COMBAT PLUS to the tank prior to adding SILWET L-77. (5) Do not allow spray mixes containing SILWET L-77 to sit over 36 hours.