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酶和辅酶 |
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R4875 | CAS:9001-99-4 | RNase A | Ribonuclease A | 核糖核酸酶A
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R4875 Ribonuclease A from bovine pancreas
Sigma Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
窗体底端
货号 号 |
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您的价格 RMB |
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R4875-100MG |
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778.05 |
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R4875-500MG |
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2,391.48 |
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R4875-1G |
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4,105.53 |
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R4875-5G |
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15,857.01 |
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Synonym: |
Pancreatic Ribonuclease, Ribonuclease I, Ribonucleate 3′-pyrimidinooligonucleotidohydrolase, RNase A |
CAS Number: |
9001-99-4 |
Enzyme Commission (EC) Number: |
3.1.27.5 ( BRENDA | IUBMB ) |
EC Number: |
232-646-6 |
MDL number: |
MFCD00132181 |
Description
Analysis Note |
Protein determined by E1%/280. |
Application |
• RNase protection assays • Remove unspecifically bound RNA • Analysis of RNA sequences • Hydrolyze RNA contained in protein samples • Purification of DNA Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. |
Preparation Note |
Salt fractionated and chromatographically purified. |
性质
type |
Type I-A |
assay |
≥60% RNase A basis (SDS-PAGE) |
form |
powder |
mol wt |
mol wt ~13,700 |
total impurities |
salt, essentially free |
foreign activity |
protease, essentially free |
storage temp. |
−20°C |
安全性
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