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R4875 | CAS:9001-99-4 | RNase A | Ribonuclease A | 核糖核酸酶A

R4875  Ribonuclease A from bovine pancreas

Sigma      Type I-A, powder, 60% RNase A basis (SDS-PAGE), 50 Kunitz units/mg protein

窗体底端

货号

您的价格
RMB

R4875-100MG

778.05

R4875-500MG

2,391.48

R4875-1G

4,105.53

R4875-5G

15,857.01

Synonym:

Pancreatic Ribonuclease, Ribonuclease I, Ribonucleate 3-pyrimidinooligonucleotidohydrolase, RNase A

CAS Number:

9001-99-4

Enzyme Commission (EC) Number:

3.1.27.5  ( BRENDA | IUBMB )

EC Number:

232-646-6

MDL number:

MFCD00132181

Description

Analysis Note Protein determined by E1%/280.
Application • RNase protection assays
• Remove unspecifically bound RNA
• Analysis of RNA sequences
• Hydrolyze RNA contained in protein samples
• Purification of DNA
Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples.
Preparation Note Salt fractionated and chromatographically purified.

性质

type Type I-A
assay ≥60% RNase A basis (SDS-PAGE)
form powder
mol wt mol wt ~13,700
total impurities salt, essentially free
foreign activity protease, essentially free
storage temp. −20°C

安全性

Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Safety Statements 22-24/25
WGK Germany 3
RTECS RF0760000