P6556 | CAS:39450-01-6 | Endopeptidase K | Proteinase K | 蛋白酶K

P6556   Proteinase K from Tritirachium album Sigma        lyophilized powder, ≥30 units/mg protein 窗体底端 货号 号 目录价 RMB P6556-5MG 299.52 P6556-10MG 358.02 P6556-25MG 444.60 P6556-100MG 604.89 P6556-500MG 2,421.90 P6556-1G 4,187.43 Synonym: Endopeptidase K CAS Number: 39450-01-6 Enzyme Commission (EC) Number: 3.4.21.64  ( BRENDA | IUBMB ) EC Number: 254-457-8 MDL number: MFCD00132129 Description Biochem/physiol Actions Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA. Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). Application Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. 性质 form lyophilized powder mol wt mol wt 28.93 kDa EQP level Premium foreign activity Dnase ≤30 Kunitz units/mg solid   RNase ≤0.003 Kunitz units/mg solid storage temp. −20°C 安全性 Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves Hazard Codes Xn Risk Statements 36/37/38-42 Safety Statements 22-24-26-36/37 WGK Germany 1