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酶和辅酶 |
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P7000 | CAS:9001-75-6 | Pepsin from porcine gastric mucosa | Pepsin 1:10000 胃蛋白酶
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P7000 Pepsin from porcine gastric mucosa
Sigma powder, 800-2,500 units/mg protein
窗体底端
货号 号 |
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您的价格 RMB |
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P7000-25G |
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463.32 |
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P7000-100G |
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1,457.82 |
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P7000-1KG |
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7,314.84 |
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Synonym: |
Pepsin A, Pepsin from hog stomach |
CAS Number: |
9001-75-6 |
Enzyme Commission (EC) Number: |
3.4.23.1 ( BRENDA | IUBMB ) |
EC Number: |
232-629-3 |
MDL number: |
MFCD00081840 |
Description
Frequently Asked Questions |
Live Chat and Frequently Asked Questions are available for this Product. |
Analysis Note |
E1%/280=14.7 |
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Protein determined by E1%/280 |
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Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH 8.0 - 8.5. |
Application |
Used to produce F(ab′)2 fragments of antibodies. |
Biochem/physiol Actions |
Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin |
Other Notes |
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer. |
Unit Definition |
One unit will produce a ΔA280 of 0.001 per min at pH2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16ml. Light path = 1cm.) |
性质
form |
powder |
mol wt |
mol wt 35 kDa |
安全性
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