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L2500 | CAS:9001-60-9 | L-Lactic Dehydrogenase | L-乳酸脱氢酶
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L2500 L-Lactic Dehydrogenase from rabbit muscle
Sigma Type II, ammonium sulfate suspension, 800-1,200 units/mg protein
窗体底端
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货号
号 |
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您的价格
RMB |
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L2500-5KU |
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464.49 |
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L2500-10KU |
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758.16 |
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L2500-25KU |
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1,605.24 |
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L2500-50KU |
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2,736.63 |
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L2500-100KU |
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4,175.73 |
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Synonym: |
(S)-Lactate: NAD+ oxidoreductase, L-LDH, LAD, LD |
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CAS Number: |
9001-60-9 |
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Enzyme Commission (EC) Number: |
1.1.1.27 ( BRENDA | IUBMB ) |
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EC Number: |
232-617-8 |
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MDL number: |
MFCD00131466 |
Description
| Analysis Note |
Protein determined by biuret. |
| Biochem/physiol Actions |
Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids. |
| Unit Definition |
One unit will reduce 1.0 μmole of pyruvate to L-lactate per min at pH 7.5 at 37 °C. |
| Physical form |
Crystalline suspension in 3.2 M (NH4)2SO4 solution, pH 6.0 |
| General description |
Lactic Dehydrogenase (LDH) has a total molecular weight of 140 kDa and is composed of 4 subunits which are designated M subunit (muscle) and H subunit (heart). These subunits may be mixed in any of 5 combinations (M4, M3H1, M2H2, MH3, and H4). Skeletal muscle contains LDH that is predominately M4 with some small amounts of M3H and traces of H2H2. The H and M subunits are quite similar in molecular weight, but differ substantially in amino acid composition. Rabbit muscle LDH dissociates into dimeric species (MW = ~70 kDa) in acetate-chloride at pH 5.0, the dissociation is reversible. Biochemistry, 13, 3527-3531 (1974). Oxidizes glyoxylate and lactate.
Isoelectric point: 8.4-8.6
Optimal pH : 7.5 . |
性质
| type |
Type II |
| form |
ammonium sulfate suspension |
| foreign activity |
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01% |
| storage temp. |
2-8°C |
安全性
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