G7141 | CAS:9001-37-0 | Glucose Oxidase | 葡萄糖氧化酶

G7141  Glucose Oxidase from Aspergillus niger Sigma       Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen) 窗体底端 货号 号 您的价格 RMB G7141-10KU 453.96 G7141-50KU 899.73 G7141-250KU 3,491.28 G7141-2.5MU 21,164.13 G7141-1MU 9,961.38 Synonym: β-D-Glucose:oxygen 1-oxidoreductase, G.Od., GOx CAS Number: 9001-37-0 Enzyme Commission (EC) Number: 1.1.3.4  ( BRENDA | IUBMB ) EC Number: 232-601-0 MDL number: MFCD00131182 Description Frequently Asked Questions Live Chat and Frequently Asked Questions are available for this Product. Analysis Note Protein determined by biuret Quality May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase. Unit Definition One unit will oxidize 1.0 μmole of β-D-glucose to D-gluconolactone and H2O2 per min at pH 5.1 at 35 °C, equivalent to an O2 uptake of 22.4 μl per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%. General description Molecular Weight: 160 kDa (gel filtration) pI: 4.2 Extinction coefficient: E1% = 16.7 (280 nm) Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation. Glucose oxidase is capable of oxidizing D-aldohexoses, monodeoxy-D-glucoses, and methyl-D-glucoses at varying rates. The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β-D-glucose with a KM of 33-110 mM. Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide. Glucose oxidase can be utilized in the enzymatic determination of D-glucose in solution. As glucose oxidase oxidizes β-D-glucose to D-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for β-D-glucose, solutions of D-glucose can be quantified as α-D-glucose will mutorotate to β-D-glucose as the β-D-glucose is consumed by the enzymatic reaction. 性质 type Type X-S form lyophilized powder composition Protein, 65-85% foreign activity Catalase ≤5 units/mg protein storage temp. −20°C 安全性 Personal Protective Equipment dust mask type N95 (US), Eyeshields, Faceshields, Gloves Hazard Codes Xn Risk Statements 42 Safety Statements 22-45 WGK Germany 1 RTECS RQ8452000