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碳水化合物 |
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43819 | CAS:3483-12-3 | DTT | DL-Dithiothreitol | 二硫苏糖醇
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43819 DL-Dithiothreitol
Fluka ≥99.0% (RT)
窗体底端
货号 号 |
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您的价格 RMB |
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43819-1G |
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476.19 |
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43819-5G |
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1,184.04 |
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43819-25G |
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4,774.77 |
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Synonym: |
threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT |
CAS Number: |
3483-12-3 |
Linear Formula: |
HSCH2CH(OH)CH(OH)CH2SH |
Molecular Weight: |
154.25 |
Beilstein Registry Number: |
1719757 |
EC Number: |
222-468-7 |
MDL number: |
MFCD00004877 |
Description
Application |
An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM). |
性质
assay |
≥99.0% (RT) |
total impurities |
≤0.3% 4,5-dihydroxy-1,2-dithiane |
mp |
41-44 °C(lit.) |
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42-43 °C |
solubility |
H2O: soluble50 mg/mL, clear, colorless |
anion traces |
sulfate (SO42-): ≤50 mg/kg |
cation traces |
Ca: ≤10 mg/kg |
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Cd: ≤5 mg/kg |
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Co: ≤5 mg/kg |
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Cr: ≤5 mg/kg |
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Cu: ≤5 mg/kg |
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Fe: ≤5 mg/kg |
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K: ≤50 mg/kg |
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Mg: ≤5 mg/kg |
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Mn: ≤5 mg/kg |
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Na: ≤200 mg/kg |
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Ni: ≤5 mg/kg |
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Pb: ≤5 mg/kg |
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Zn: ≤5 mg/kg |
storage temp. |
2-8°C |
安全性
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