G5013 | CAS:108321-42-2 | G 418 disulfate salt

G5013  G 418 disulfate salt Sigma      potency: ≥450 μg per mg (anhydrous basis) 窗体底端 货号 号 目录价 RMB G5013-250MG 347.49 G5013-1G 803.79 G5013-5G 3,545.10 Synonym: Antibiotic G418 CAS Number: 108321-42-2 Empirical Formula (Hill Notation): C20H40N4O10 · 2H2SO4 Molecular Weight: 692.71 MDL number: MFCD00058314 PubChem Substance ID: 24895193 Description Biochem/physiol Actions Mode of Action: Blocks polypeptide synthesis by inhibiting protein elongation. For use in the selection and maintenance of eukaryotic cells stably transfected with neomycin resistance genes. Caution Stable at 37 °C for 8 days. Other Notes Aminoglycoside antibiotic similar in structure to gentamicin, neomycin and kanamycin. Reconstitution Aqueous solutions stored frozen at -20 to -70 °C should be stable for approximately six months. Application Blocks protein synthesis by interfering with ribosomal function. Not normally used as a standard antibiotic, but rather for selection and maintenance of prokaryotic and eukaryotic cells stably transfected with neomycin resistance genes. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. The resistance genes are APH (3′) II or aminoglycoside phosphotransferase 3′(II) and aminoglycoside phosphotranferase 3′ (I) or APH (3′) I. The following concentrations of active ingredient may be used as a guide for selection: mammalian cells (400 ug/ml), plants (10 ug/ml), yeasts (500 ug/ml), and dictyostelium (10 ug/ml). Cells have variable resistance and multiplying cells will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. Varying concentrations should be tested. Additionally, fibroblast contamination in mixed cultures may be suppressed by using 100 ug/ml of the active ingredient for 2 days. Protocols & Applications Antibiotic Selector for application, solubility, solution stability, working concentration, and mode of action information 性质 form white powder potency ≥450 μg per mg (anhydrous basis) solubility H2O: soluble50 mg/mL storage temp. 2-8°C 安全性 Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter Safety Statements 22-24/25 WGK Germany 3